Functional conservation of multiple elements in yeast chromosomal replicators
نویسندگان
چکیده
منابع مشابه
Multiple functional elements comprise a Mammalian chromosomal replicator.
The structure of replication origins in metazoans is only nominally similar to that in model organisms, such as Saccharomyces cerevisiae. By contrast to the compact origins of budding yeast, in metazoans multiple elements act as replication start sites or control replication efficiency. We first reported that replication forks diverge from an origin 5' to the human c-myc gene and that a 2.4-kb ...
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Although the yeast his3 promoter region contains two functional TATA elements, TR and TC, the GCN4 and GAL4 upstream activator proteins stimulate transcription only through TR. In combination with GAL4, an oligonucleotide containing the sequence TATAAA is fully sufficient for TR function, whereas almost all single-base-pair substitutions of this sequence abolish the ability of this element to a...
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A yeast autonomously replicating sequence, ARS305, shares essential components with a chromosome III replicator, ORI305. Known components include an ARS consensus sequence (ACS) element, presumed to bind the origin recognition complex (ORC), and a broad 3'-flanking sequence which contains a DNA unwinding element. Here linker substitution mutagenesis of ARS305 and analysis of plasmid mitotic sta...
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The Saccharomyces cerevisiae RAD54 gene has critical roles in DNA double-strand break repair, homologous recombination, and gene targeting. Previous results show that the yeast gene enhances gene targeting when expressed in Arabidopsis thaliana. In this work we address the trans-species compatibility of Rad54 functions. We show that overexpression of yeast RAD54 in Arabidopsis enhances DNA dama...
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Replicators don't. Replicate, that is. This is the shocking conclusion to which I have been forced by my attempt to gure out what precisely Richard Dawkins means by the term \replicator". Actually, it seems that Dawkins uses the term in at least two fundamentally diierent ways; but according to Dawkins' own speciication of the problem which the \replicator" concept was intended to solve (namely...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1994
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.14.11.7643-7651.1994